Identification of eosinophils by flow cytometry

Detection of eosinophils in whole blood samples by flow cytometry.

A flow cytometric method to detect and study human eosinophils in whole blood was established. Normal subjects and patients with various types of eosinophilia (hypereosinophilic syndromes, allergic diseases, dermatitis, Hodgkin's Disease, parasitosis) were studied. Whole blood samples were treated for 10 minutes at room temperature with a commercially available reagent (FACS Lysing Solution, Be...

Module : Antibody detection / identification – by Flow Cytometry

Identification and Characterization of Bacillus anthracis Spores by Flow Cytometry

Rapid and accurate detection of Bacillus anthracis, the causative agent of anthrax, remains an active area of research due to the continued threat of bioterrorist attack. The ability to differentiate Bacillus anthracis spores from spores belonging to other Bacillus species is important for the development of spore-based detection methods. Furthermore, not all Bacillus anthracis strains are full...

Detection, Identification and Susceptibility Testing of Bacteria by Flow Cytometry

With the multiparametric measurement of individual cells at a rate of several thousand per second, flow cytometry offers a rapid method for the detailed analysis of microorganisms. This technique provides quantitative data for cell size or granularity through light scattering signals and via fluorescence signals quantitative information on the expression of surface antigens, or intracellular co...

Evaluation of DNA-content of Peritoneal Lavage Cells by Flow Cytometry in Patients with Gasteric Cancer

Background & Aims: Gastric cancer is the second common cancer in Iran and it has unfortunately no diagnostic signs in early stages. Considering the role of genetic and environmental factors in the development of gastric cancer, this study aimed at evaluating DNA-content of stomach cells in patients with gastric cancer in order to find a reliable index for prediction of disease prognosis and pro...